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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 205
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Cell_line_name |
2M6
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Brief_description |
confirmed as mouse with IEF of LDH, NP cell line was established by fusion of
mouse NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-
purified chicken retina E10 proteins; described as secreting a mouse monoclonal
antibody (IgG) reacting with chicken retina (Mueller glia cell-specific antigen)
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Description |
mouse hybridoma (anti-chicken retina) cell line was established by fusion of mouse
NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-purified
chicken retina E10 proteins; described as secreting a mouse monoclonal antibody
(IgG) reacting with chicken retina (Mueller glia cell-specific antigen) confirmed as
mouse with IEF of LDH, NP
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Depositor |
Prof. B. Schlosshauer, Natural and Medical Science Institute (NMI), Reutlingen,
Germany
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Morphology |
round cells growing singly or in small clumps in suspension, some cells are slightly
adherent
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Culture_conditions |
80-85% RPMI 1640 + 10-15% FBS + HFCS ("Hybridoma Fusion and Cloning
Supplement") for the first 1-2 weeks post-thawing cells are difficult to culture! maintain
at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; seed out
at ca. 0.2 x 106 cells/ml; after thawing cultivate cells for the first 1-2 weeks in 24- or
12-well-plates using HFCS at 37C with 5% CO2 maximal density at ca. 0.8 x 106
cells/ml
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Viruses |
ELISA: reverse transcriptase positive; PCR: SMRV -
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Properties |
secretion of IgG 1 monoclonal antibody
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule
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Doubling_time |
ca. 35 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics murine hypertetraploid karyotype
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Bibliographic_reference |
Schlosshauer et al., Development, 111: 19 (1991) [PMID: 1879342
] |
Schlosshauer et al., Brain Res Dev Brain Res, 59: [PMID: 1914140]